INTENDED USE OF NICOTINE URINE TEST
(COT) Test is a lateral flow, one-step immunoassay for the qualitative detection of Cotinine, the principle metabolite of nicotine, in human urine at a cut-off sensitivity level of 200 ng/ml. Cotinine is a principle and residual metabolite of Nicotine which remains in the body of habitual tobacco users. The nicotine urine test is intended for forensic, non-diagnostic use only to determine tobacco or nicotine use.
Most experts and healthcare professionals agree that nicotine is unquestionably the most addictive drug in use today. The principle source of nicotine is tobacco products. Tobacco smoking or chewing results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into 20 metabolites excreted in urine. Cotinine, a major metabolite of nicotine accumulates in the body with regular smoking. It is reported that Cotinine is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites. Cotinine has been widely used as a bio-marker of tobacco exposure. Methods of analysis for Cotinine in biological fluids include gas chromatography, gas chromatography-mass spectrometry, HPLC, HPLC-mass spectrometry, EIA and RIA. These methods usually require special equipment and complicated operation procedures. For comprehensive information on drug testing including typical half-life or drug detection periods see the Drug Testing FAQ.
The One-Step Rapid Nicotine Urine Test is a one-step immunoassay that is used for the qualitative detection of Cotinine in human urine. It is based on the principle of highly specific immunochemical reactions of antigens and antibodies. This urine test for nicotine is a simple and convenient test for the rapid qualitative detection of Cotinine in human urine at 200 ng/ml cut-off concentration.
The One-Step Rapid Nicotine Urine Test applies the principle of competitive immunoassay. The urine nicotine test device contains a membrane strip that is pre-coated with Cotinine antigen at the test line region. The Cotinine antibody gold conjugate pad is placed at the end of the membrane. In Cotinine-free urine, the colored antibody-colloidal gold conjugate and urine moves chromatographically by capillary action across the membrane. This solution migrates to the test line containing Cotinine antigen and forms a visible line as the antibody complexes with the antigen. The formation of a visible precipitant in the test zone indicates a negative result (non-smoker). When Cotinine is present in urine, it competes with Cotinine on the test band region for the limited antibody sites. When a sufficient concentration of Cotinine is present in urine, it will compete at the limited antibody binding sites. This will prevent attachment of the colored antibody-colloidal gold conjugate at the test line region. Therefore, absence of the color band on the test region indicates a positive result (smoker).
Individually sealed nicotine urine test device which includes one disposable pipette each and complete instruction sheet with test technology, inter-reaction and cross-reaction data.
INTERPRETATION OF NICOTINE URINE TEST RESULTS:
(1) pink-rose band appears in the control zone and no band appears in the test zone.
A positive result indicates the Cotinine level is 200 ng/ml or higher in the test urine
The One-Step Rapid Nicotine (COT) Test is available to professionals and individuals concerned with tobacco use/abuse. The nicotine test kit is compact, self-contained, individually packaged and can be performed any time the targeted drug use is suspected. The tests come in minimum order units of five (5) individual tests at a cost per single test screen of $4.50 for the minimum order unit.